Phytochemical screening, Biochemical Parameters and Histopathological Changes in Liver of Albino Rats Orally Dosed with (Leptadenia pyrotechnica (Marakh) Ethanolic
Keywords:
leptadenia pyrotechnica, Phytochemical screening, Biochemical Parameters, Histopathological Changes
Abstract
leptadenia pyrotechnica plant traditionally used in folkloric medicine in Sudan to cure of cough, fever, kidney disorders, stones and urinary disease. The plant tested for its secondary metabolites and toxic effects, the results exhibited high presence of saponins, alkaloids, tannins and triterpens, moderate presence of flavonoids, and negative presence of cumarins and steroids. Rats which dosed with plant extract showed significant differences for Alkaline Phosphate (ALP), albumin and bilirubin levels (p<0.01), while the other parameters represented no significant changes from the control. Histological examination of the plant showed swelling, dilated central vein and peripheral hepatocytes vacoulation.
References
Afolayan, A. J. and Yakubu, M.T. (2009). Effect of Bulbine natalensisbark stem extract on the functional indices and histology of the liver and kidney of male Wistar rats. Journal of Medicinal Food 12 (4): 814-820.
Alqasoumi, S.I., Soliman, G.A.E.H., Awaad, A.S. and Donia, A.E.R.M. (2012). Anti-inflammatory activity, safety and protective effects of Leptadenia pyrotechnica, Haloxylon salicornicum and Ochradenus baccatus in ulcerative colitis. Phytopharmacology, 2(1), pp.58-71.
Ashafa, A., Sunmonu T. and Afolayan, A. (2010). Toxicological evaluation of aqueous leaf and berry extracts of Phytolacca dioical in male Wistar rats. Food Chem. Toxicol., 48, 1886-1889
Firenzuoli, F. and Cori, L. (2007). Herbal medicine today: Clinical and research issues. Evd. Based Complement. Alternat. Med., 4, 37-40.
Fransworth, N. (1994). Ethanopharmacology and drug development. In: Ethanobotany and the search for new drugs, Ciba foundation symposium 185 (Prance, G. & March, J. eds.). John Wiley and sons, Chichester, P.42-59.
Gomez, K. and Gomez, A., (1984). Statistical procedures for agricultural research 2nd Ed. John Wiley and Sons, Inc. New York.
Harborne, J. B. (1984). Phytochemical methods. 2nd edition. Chapman and Hall.
Hostettmann, K. and Maston, A. ( 2002). Twenty years of research into medicinal plants: Results and perspectives. Phytochem. Rev., 1, 275-285
Idrees, S., Qureshi, R., Bibi, Y., Ishfaq, A., Khalid, N., Iftikhar, A. and Ahmad, N. (2016). Ethnobotanical and biological activities of Leptadenia pyrotechnica (Forssk.) Decne. A review. African Journal of Traditional, Complementary and Alternative Medicines, 13(4), 88-96.
Martinez A, Valencia G: Marcha fitoquimica. (2003). In Manual de prácticas de Farmacognosia y Fitoquímica: 1999. 1. st edition. Medellin: Universidad deAntioquia; Phytochemical screening methods 59-65.
Munazir, M., Qureshi, R. and Munir, M. (2015). Preliminary phytochemical screening of roots and aerial parts of Leptadenia pyrotechnica. Pak. J. Bot, 47(2), pp.659-664.
Ouedraogo, Y., Guissou, I. and Nacoulma, O. ( 2007). Biological and toxicology study of aqueous root extract from Mitragyna inermis (Willd Oktze) Rubiaceae. Int. J. Pharmacol., 3, 80-85.
Qureshi, R., Munazir, M., Abul-Soad, A. A., Jatoi, M. A. and Shabbir, G. (2012). In vitro callus induction protocol for Leptadenia pyrotechnica using various explants. Journal of Medicinal Plants Research, 6(3), 379-382.
Shyamal, S., Latha, P.G., Suja, S.R., Shine, V.J., Anuja, G.I., Sini, S., Pradeep, S., Shikha, P. and Rajasekharan, S. (2010). Hepatoprotective effect of three herbal extracts on aflatoxin BI- intoxicated rat liver. Singapore Med. J. 5(4) : 326-331.
Sofowora, A. (1993). Medicinal Plants and Traditional Medicines in Africa.
Chichester John, Willey & Sons New York 256.
Soliman, G. A., Donia, A. E. R. M., Awaad, A. S., Alqasoumi, S. I., & Yusufoglu, H. (2012). Effect of Emex spinosa, Leptadenia pyrotechnica, Haloxylon salicornicum and Ochradenus baccatus extracts on the reproductive organs of adult male rats. Pharmaceutical biology, 50(1), 105-112.
Verma, N., Jha, K. K., Chaudhary, S., Singh, O., & Kumar, A. (2014). Phytochemistry, pharmacology and traditional uses of Leptadenia pyrotechnica-An important medicinal plant. Indian Journal of Pharmaceutical and Biological Research, 2(01), 128-134.
Wintola, O., Sunmonu, T. and Afolayan, A. (2010). The effect of Aloe ferox MIII. in the treatment of loperamide-induced constipation in Wistar rats. BMC Gastroenterol, 10, 95.
Watafua, M. and Geidam, M.A. (2014). Effect of ethanolic extract of aerials of Leptadenia pyrotechnica (Foskk) Dence on the liver function of Wister strain Albino rats. Ent. J. Scientific research and management. 2(3), 3221-3418.
Youssef Moustafa, A.M., Khodair, A.I. and Saleh, M.A. (2009). Isolation, structural elucidation of flavonoid constituents from Leptadenia pyrotechnica and evaluation of their toxicity and antitumor activity. Pharmaceutical Biology, 47(6), 539-552.
Alqasoumi, S.I., Soliman, G.A.E.H., Awaad, A.S. and Donia, A.E.R.M. (2012). Anti-inflammatory activity, safety and protective effects of Leptadenia pyrotechnica, Haloxylon salicornicum and Ochradenus baccatus in ulcerative colitis. Phytopharmacology, 2(1), pp.58-71.
Ashafa, A., Sunmonu T. and Afolayan, A. (2010). Toxicological evaluation of aqueous leaf and berry extracts of Phytolacca dioical in male Wistar rats. Food Chem. Toxicol., 48, 1886-1889
Firenzuoli, F. and Cori, L. (2007). Herbal medicine today: Clinical and research issues. Evd. Based Complement. Alternat. Med., 4, 37-40.
Fransworth, N. (1994). Ethanopharmacology and drug development. In: Ethanobotany and the search for new drugs, Ciba foundation symposium 185 (Prance, G. & March, J. eds.). John Wiley and sons, Chichester, P.42-59.
Gomez, K. and Gomez, A., (1984). Statistical procedures for agricultural research 2nd Ed. John Wiley and Sons, Inc. New York.
Harborne, J. B. (1984). Phytochemical methods. 2nd edition. Chapman and Hall.
Hostettmann, K. and Maston, A. ( 2002). Twenty years of research into medicinal plants: Results and perspectives. Phytochem. Rev., 1, 275-285
Idrees, S., Qureshi, R., Bibi, Y., Ishfaq, A., Khalid, N., Iftikhar, A. and Ahmad, N. (2016). Ethnobotanical and biological activities of Leptadenia pyrotechnica (Forssk.) Decne. A review. African Journal of Traditional, Complementary and Alternative Medicines, 13(4), 88-96.
Martinez A, Valencia G: Marcha fitoquimica. (2003). In Manual de prácticas de Farmacognosia y Fitoquímica: 1999. 1. st edition. Medellin: Universidad deAntioquia; Phytochemical screening methods 59-65.
Munazir, M., Qureshi, R. and Munir, M. (2015). Preliminary phytochemical screening of roots and aerial parts of Leptadenia pyrotechnica. Pak. J. Bot, 47(2), pp.659-664.
Ouedraogo, Y., Guissou, I. and Nacoulma, O. ( 2007). Biological and toxicology study of aqueous root extract from Mitragyna inermis (Willd Oktze) Rubiaceae. Int. J. Pharmacol., 3, 80-85.
Qureshi, R., Munazir, M., Abul-Soad, A. A., Jatoi, M. A. and Shabbir, G. (2012). In vitro callus induction protocol for Leptadenia pyrotechnica using various explants. Journal of Medicinal Plants Research, 6(3), 379-382.
Shyamal, S., Latha, P.G., Suja, S.R., Shine, V.J., Anuja, G.I., Sini, S., Pradeep, S., Shikha, P. and Rajasekharan, S. (2010). Hepatoprotective effect of three herbal extracts on aflatoxin BI- intoxicated rat liver. Singapore Med. J. 5(4) : 326-331.
Sofowora, A. (1993). Medicinal Plants and Traditional Medicines in Africa.
Chichester John, Willey & Sons New York 256.
Soliman, G. A., Donia, A. E. R. M., Awaad, A. S., Alqasoumi, S. I., & Yusufoglu, H. (2012). Effect of Emex spinosa, Leptadenia pyrotechnica, Haloxylon salicornicum and Ochradenus baccatus extracts on the reproductive organs of adult male rats. Pharmaceutical biology, 50(1), 105-112.
Verma, N., Jha, K. K., Chaudhary, S., Singh, O., & Kumar, A. (2014). Phytochemistry, pharmacology and traditional uses of Leptadenia pyrotechnica-An important medicinal plant. Indian Journal of Pharmaceutical and Biological Research, 2(01), 128-134.
Wintola, O., Sunmonu, T. and Afolayan, A. (2010). The effect of Aloe ferox MIII. in the treatment of loperamide-induced constipation in Wistar rats. BMC Gastroenterol, 10, 95.
Watafua, M. and Geidam, M.A. (2014). Effect of ethanolic extract of aerials of Leptadenia pyrotechnica (Foskk) Dence on the liver function of Wister strain Albino rats. Ent. J. Scientific research and management. 2(3), 3221-3418.
Youssef Moustafa, A.M., Khodair, A.I. and Saleh, M.A. (2009). Isolation, structural elucidation of flavonoid constituents from Leptadenia pyrotechnica and evaluation of their toxicity and antitumor activity. Pharmaceutical Biology, 47(6), 539-552.
Published
2022-12-29
Section
Articles
