Invivo and Invitro of Trichoderma harzianum, Bacterial Strains and Imazethapyr on Orobanche crenata Forsk on Faba bean Vicia faba

Abstract

A series of experiments were undertaken at the laboratories and experimental farm of Environment, Natural Resources and Desertification Research Institute, National Center for Research (NCR) and Shendi Research Station experimental farm, Agricultural Research Corporation (ARC), at season 2015-2016, to evaluate efficacy of Trichoderma harzianum, Bacillus megatherium var. Phosphaticum (BMP), Rhizobium leguminosarum (TAL1399) and the herbicide pursuit 10% SL (imazethapyr) against Orobanche crenata infesting faba bean. In laboratory experiments treatments were laid out in a Completely Randomized Design (CRD) and in field experiments a Randomized Complete Block Design (RCBD) was employed with four replicates. Results of laboratory experiments showed that T. harzianum and Imazethapyr each alone or in combination significantly reduced O. crenata germination. Field results revealed that, application of T. harzianum; Imazethapyr and Imazethapyr + BMP + TAL1399 significantly delayed the days of O .crenata emergence in Shendi and Soba sites. T. harzianum alone or in combination with bacteria or imazethapyr significantly reduced number of O. crenata emergence and increased faba bean plant height as compared to the corresponding control in Shendi and Soba sites. T. harzanium alone or in combination with T. harzanium + BMP + TAL1399 significantly increased faba bean biomass, pods/plant, grain yield and 100 seed weight as compared to the infested control in Shendi and Soba sites.