Biochemical Effects of Tribulus-Terrestuis on Ethylene glycol-induce Nephrolithiasis in Albino rats
Abstract
The present study was conducted at the Department of Biochemistry-Faculty of Veterinary Medicine University of Khartoum to investigate the effect of Preventive and Treatment dose of aqueous extract of Tribulus terrestris on induced kidney stone in albino rats. Kidney stone was induced by ethylene glycol 0.75%v/v ammonium chloride. Forty rats were divided into 4 groups I, II, III and IV, 10 rats each. Group I was saved as negative control –ve (normal rats), group II, III and IV induced kidney stone for ten days. Group II was saved as positive control +ve, groups III received 1.5ml/100gm b/w aqueous extract of T. terrestris simultaneously with induction from day one to day ten (preventive dose), group IV dose of 1.5 ml/100gm b/w aqueous extract T. terrestris from day ten to day twenty(treatment dose). Concentrations of Urea, Creatinine, Phosphorous and Calcium were measured relative to (-ve) control group and (+ve) control group.The study revealed that in preventive dose the concentrations of Urea, Creatinine, Phosphorous and Calcium were significantly (P˂0.05) reducedin treated group compared to (+ve) control group by 56.5%, 21.5%, 58.9%, 3.16% respectively. No significant change in the creatinine between treated and –ve control, urea and phosphorous were significantly (P˂0.05) decreased while calcium was significantly (P˂0.05) increased between treated group compared to -ve control. In treated dose urea, creatinine and phosphorous were reduced significant (P˂0.05) but no significant change in calcium compare to +ve control by 56.88%, 68.9%, 42.24% respectively. There were no significant change in urea, creatinine and phosphorous between the treated and negative control. However the level of calcium in treated group was significantly increase (P˂0.05) compared to negative control.
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