Antimicrobial Potential of Acacia nilotica subsp. tomentosa Against Some microorganisms causes Urogenital Infections.

  • Khalid Suliman Abd-Ulgadir
Keywords: Antimicrobial potential; Acacia nilotica subsp. tomentosa; leaves extracts; Bark extract; Antifungal activity.

Abstract

Background: For centuries, the therapeutic properties of various medicinal plants have been used to treat human illnesses. Aim: This study was aimed to investigate and compare the antimicrobial properties of leaves and stem bark extracts of Acacia nilotica ssp. tomentosa against standard strains and clinical isolates. Methodology: Antimicrobial activities were detected by the agar well diffusion method and the broth dilution method. The MDIZs were analyzed statistically using One- Way analysis of variance using SPSS Version (20). Results: Methanol extracts of leaves and stem bark exhibited unique antimicrobial activity against all standard strains of bacteria and fungi with zones of inhibition ranged from 21 mm to 39 mm. The leaves and stem bark aqueous extracts were also showed high activity on the standard strains of bacteria and fungi showing diameters of inhibition zones which were less than methanol extracts and was ranged from 20 mm to 23 mm. The MIC showed by leaves and bark methanol extracts on the standard bacteria was ranged from 3.125 mg/ml to 12.50 mg/ml and the MBC was ranged from 6.25 mg/ml to 50 mg/ml. On the other hand, the MIC of leaves and bark methanol extracts toward standard fungi was 6.25 mg/ml, while MFC was 12.50 mg/ml. Leaves and bark methanol extracts revealed significant antimicrobial activity on the bacterial isolates viz: Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus saprophyticus,    Neisseria  gonorrhoeae,   Klebsiella  pneumoniae  and Enterococcus  spp. where the MDIZ±SD was ranged from 22.36±7.40 mm to 25.54±2.47 mm. On the other hand, isolates of Candida albicans showed MDIZ which was 23.20±3.44 mm for leaves methanol extract and 25.25±1.92 mm for bark methanol extract. The MIC exhibited by leaves and bark methanol extracts on bacterial isolates was ranged from 3.125 mg/ml to 12.50 mg/ml and the MBC was ranged from 6.25 mg/ml to 25 mg/ml. The MIC from the same extracts toward clinical isolates of Candida albicans was 6.25 mg/ml, whereas the MFC was 12.50 mg/ml. Conclusion: The study revealed that the extracts of A. n. ssp. tomentosa possess significant antimicrobial compounds, which proved to be effective against both standard strains and clinical isolates.

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Published
2019-09-15