RP – HPLC Method development and validation of Sodium valproate in pharmaceutical formulations

الملخص

A rapid, simple, and sensitive HPLC method developed for optimization, validation and determination of sodium valporate tablet in two concentrations. Stability study, chromatographic conditions comprised of a 250 X 4.6 mm column containing 5-µm, packing L₁ (C₁₈), mobile phase consisting of a mixture of 35 volumes of buffer and 65 volumes of acetonitrial with flow rate of 1 mL/min. Detection at 220 nm. The retention time of sodium valporet was 3.35 min. The linear regression analysis data for the calibration plots showed good linear relationship within the concentration range 250-1500 µg/mL. The value of correlation coefficient was found to be 0.999149. The recovery of sodium valproate was 98–102%. The method was validated as per International Council for Harmonization (ICH) guidelines