GC/MS Determination of Compounds from n-Hexane Extract of Leaf Cuticular Wax in Polygonum glabrum Willd.

  • Hatil Hashim EL-Kamali Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
  • Aboud Mubark Mohammed Ahmed Department of Chemistry; Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
  • AL-Siddig Mohammed AL-Siddig Department of Chemistry; Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
  • Burhan Abdi Nor Department of Chemistry; Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
  • Muhanad EL-Fatih Abdalla
  • Nour EL-Din Ahmed Abdalla Department of Chemistry; Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
Keywords: chemical composition, leaf cuticular wax, Polygonum glabrum, Sudan

Abstract

Epicuticular waxes play a role in plant biology in water loss regulation as the cuticle acts as a barrier to water vapor, influencing cuticular transpiration and as a protective barrier against environmental humidity fluctuations. Chemical composition of leaf epicuticular wax was studied in Polygonum glabrum Willd. The leaf epicuticular wax was obtained by cold extraction in n-hexane and then analyzed by Gas Chromatography / Mass spectrometry (GC/MS). The principal components of the wax were alcohols, fatty acids and n-alkanes. The most abundant compounds were 6,6-dimethyl-1,3-heptadien-5-ol (11%), oleic acid (10.59%), 9-octadecenamide, (Z)- (9.85%), 1,2,3-propanetriol diacetate (9.35%) and Octacosane (6.25%). These results provide the first report of the n-hexane–soluble epicuticular wax composition of P. glabrum, contributing to our understanding of its ecological adaptations and potential bioactive properties.

References

- Ezhilan B. and Neelamegam R. (2011). GC/MS Determination of bioactive compounds of Polygonum glabrum (Willd.). Journal of Phytology 3(9): 23-25.
- Jacobson U and Muddathir A. (1992). Four biologically active sesquiterpenes of the drimane type isolated from Polygonum glabrum Phytochemistry 31(12):4207 4211.
- Kiron SS, Nizar K, Rajagopal PL, Saritha M, Narayaswamy VB (2012). Analgesic activity study of Polygonum glabrum Willd in rodents. Research Journal of Pharmaceutical , Biological and Chemical Science. 3(3): 1157.
- Koche DK, Shirsat RP, Syed Imran, Mohd. Nafees, Zingare AK, Donode KA. Ethnomedicinal Survey of nagzira wild life sanctuary, District Gondia (M.S.) India-Part II. Ethnomedicinal Leaflets. 2008; 1(8): 532-537.
- Medina E, Aguiar G, Gomez M, Aranda J , Medina D and Winter K (2006). Biochemical systematic and Ecology , 34, 321.
- Mohammed IS (1996). Phytochemical studies of flavonoids from Polygonum glabrum L. of Sudan. MSc thesis , Chemistry , University of Khartoum.
- Neelamegam, R. (2015). Phytochemical analysis and biological activities of Polygonum glabrum (Timsahiya). Journal of Medicinal Plants Research, 9(12), 442-448.
- Neinhuis C, Barthlott W (1997). The tree leaf surface: structure and function. In Ziegier H (eds.) Trees- Contributions to modern tree physiology. Backhuys. Leiden, Netherland. Pp. 3-18.
- Schreiber L, Riederer P E (1996). Ecophysiology of cuticular transpiration ; comparative investigation of cuticular water permeability of plant species fromdifferent habitats. Oecologia 107:426-432.
- Shankar LH, Mishra PK. Study of aquatic medicinal plants of Hazaribagh district of International Jharkhand, India. research journal of pharmacy. 2012; 3(4): 405 409.
- Singh B, Pandey VB, Joshi VK and Gambhir SS (1987). Antiinfalmmatory action of Polygonum glabrum. J. Ethnopharmacology 19(3):255-267.
- Sivakumar P., Senthilkumar K.L. , Varma J.P. (2011). Phytochemical Studies on Plygonum glabrum Willd. International Journal of Pharma and Biosciences 2(2):169.
Published
2025-09-20
How to Cite
Hatil Hashim EL-Kamali, Aboud Mubark Mohammed Ahmed, AL-Siddig Mohammed AL-Siddig, Burhan Abdi Nor, Muhanad EL-Fatih Abdalla, & Nour EL-Din Ahmed Abdalla. (2025). GC/MS Determination of Compounds from n-Hexane Extract of Leaf Cuticular Wax in Polygonum glabrum Willd. Journal of The Faculty of Science and Technology, 10(10), 1 - 8. https://doi.org/10.52981/jfst.v10i10.3400