Comparative study of formal ether concentration, wet preparation, and zinc sulphate flotation techniques for intestinal parasite diagnosis. Omdurman State-Sudan

Jamal Yousef Lowaty   Lowba; Mohamed  Abdurrahman; Ali Adam  Artmby; Mazen  Ahmed; Mohamed Ebrahem  Suleiman; Ismael Abu-Bakr  Ismael; Hozeefa Hasaean  Alnwor; Mohanad Elhadi  elfadol

doi:10.52981/smlj.v10i2.2846

Jamal Yousef Lowaty Lowba Omdurman Islamic University, Faculty of Medical Laboratory science
Mohamed Abdurrahman Omdurman Islamic University, Faculty of Medical Laboratory science
Ali Adam Artmby Omdurman Islamic University, Faculty of Medical Laboratory science
Mazen Ahmed Omdurman Islamic University, Faculty of Medical Laboratory science
Mohamed Ebrahem Suleiman Omdurman Islamic University, Faculty of Medical Laboratory science
Ismael Abu-Bakr Ismael Omdurman Islamic University, Faculty of Medical Laboratory science
Hozeefa Hasaean Alnwor Omdurman Islamic University, Faculty of Medical Laboratory science
Mohanad Elhadi elfadol Omdurman Islamic University, Faculty of Medical Laboratory science

DOI: https://doi.org/10.52981/smlj.v10i2.2846

Keywords: Comparison, formal ether, Zinc sulphate, flotation Techniques, wet preparation, intestinal parasites

Abstract

background. The faecal formal ether concentration technique has become a routine method as a part of complete parasite examination. It allows the detection of small organisms that may be missed by using zinc sulphate flotation and direct microscopy techniques.

Objective: The study aimed to compare between the reactivity of Formal ether, zinc sulphate flotation and direct microscopy techniques in detecting parasites ova, cysts, and larvae in the stool.

Material and Methods: This descriptive-based case study was conducted during the period from September to December 2021 in Ombada and Basher hospital in Omdurman - state. A total of 150 stool samples were collected and preserved in 10% formal saline. All samples were examined using wet preparation, Zinc sulphate flotation and formal ether concentration techniques.

Results. Out of 150 stool specimens examined, 104 cases were found to be positive in the deferments techniques used. The zinc sulfate flotation technique parasites detected was (28%), the formal ether technique parasites detection rate was (40%) and the wet preparation detection rate was (36%). The types of parasites detected in this study using different techniques were, E. histolytic (34%), Giardia lamblia 43.7%, Ascaris 0%, H. nana 33%, Taenia SPP 50% detected by formal ether technique, parasites detected by using wet preparation techniques were Giardia lamblia 31.3%, Ascaris 1%, Taenia SPP 50%, H. nana 33 % and E. histolytica 29%, Giardia. lamblia 25% was detected by using the Zinc sulphate technique.

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