Effect of Campylobacter Coli Lipopolyssccharides on Phagocytosis in Vitro and in Vivo

Haitham E M Elawad

Haitham E M Elawad

Keywords: C. coli, LPS, Phagocytosis

Abstract

Campylobacter coli usually live in the intestinal tract of animals and cause inflammation of the intestine and diarrhea. In this study, C. coli were isolated from patients having diarrhea and identified based on morphological and biochemical characteristics. Lipopolysaccharide (LPS) is major outer membrane structural component of gram negative bacteria which induces a broad range of biological response. Here, LPS was isolated with hot phenol-water extraction and purified by gel filtration using Sephadex G-200. Phagocytosis is essential for fighting infections in immune responses to protect organisms from bacteria invasion hence we studied the effect of purified LPS on phagocytosis both in vitro and in vivo. The results reported in this study may prove to be valuable in analyzing the LPS effect on host immune response.

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