Oral Toxicity of Agro-Fungicides: Tilt (Propiconazole), Bayleton (Triadimefon) And Their Mixture to Nubian Goats
Abstract
Introduction: The hazard use of pesticides, emergence of many diseases with high prevalence e.g (cancer, kidney failure and hepatic problems) urged the need for research on fungicides which are continuously received by human in Sudan via fruit and vegetables.
Objective: To detect the toxicity of these fungicides in experimental animals.
Methods: Twelve Nubian goats were used in these experiments; they were grouped into three groups (and one control group) and dosed orally with two fungicides [Propiconazole (100mg/kg/day), Triadimefon (100mg/kg/day)] and their mixture (50:50 mg/kg/day). Animals were closely observed for clinical signs and behavior. Dead or slaughtered animals underwent postmortem examination and lesions were recorded. Samples from different organs were preserved for histopathological studies. Fresh
blood was collected for heamatological and Serobiochemical analysis.
Results: Five minutes post-dosing, the animals showed some clinical signs which recovered after four hours. Death occurred in days 12- 25 in the animals dosed with the mixture. The most prominent feature in postmortem lesions was the congestion in different organs. Histopathological changes were the fatty change of liver and kidneys. In Triadimefon dosed group, the values of PCV, Hb and MCHC decreased significantly (p<0.001). The serum urea concentration and GOT activity were high (p<0.001) in both of them. Animals dosed with mixture had significantly (p<0.05-0.001) higher PCV, MCV, MCH and WBC than the control. Significantly high values of serum urea concentration (p<0.01) and GOT activity (p<0.001) were reported in goats dosed with the mixture.
Conclusion: Both fungicides and their mixture showed toxicological and pathological effects in dosed animals.
References
Hakoi , H; Gabral, R; Hoshia, T; Hasegawa, R; Shirai, T and Jto, N. Analysis of some pesticides in a medium-term liver bioassay in the rat. Tertog-CarcinogMutagen. 1992;12:(6): 269-276
Chen, P.-J., Moore, T., and Nesnow, S. Cytotoxic Effects of Propiconazole and Its Metabolites in Mouse and Human Hepatoma Cells and Primary Mouse Hepatocytes. 5th Society of Environmental Toxicology and Chemistry (SETAC) World Congress, Australia, Sydney, August 3-7. 2008.
Plant Protection Directorate, Department of Pesticide. Annual Report (1998). Plant Protection Directorate, Department of Pesticide, Ministry of Agriculture, Sudan.1998.
EPA (Environmental Protection Agency. Pesticide tolerance for 1(2-(2, -4 dichlorophenoxyl) 4- propyl 1, -3 dioxolan -2- YL (methyl) 1-h-1, 2,4- triazole and its metabolite known as Propiconazole. Washington USA.1999.
Bayer (ed.) . Triadimefon systemic fungicide, technical information. 1985; pp27.
Crofton, K.M. A structure-activity relationship for the neurotoxicity of triazole fungicides: Toxil-Lett. 1996; 84(3):155-159.
U.S. Department of Agriculture, Forest Service. Propiconazole pesticide fact sheet. Information Ventures Inc. U.S.A. 1999.
Hyes, W.J. Toxicology of Pesticides. The Williams & Wilk Company .USA (Pupl.).1985.
Mahaney, R.C. Laboratory Techniques in Zoology. White Friars ltd. London. 1983.
Byrkit, D.R. Statistics today, a comprehensiveintroduction. The Benjamin / Cummings Publishing Company, California.1987; P.P.428-476.
Crofton, K.M; Ronek,V.M and MacPhail, R.C. Evidences for monoaminergic involvement to Triadimefon induced hyperactivity. Psyhopharmacology. Berl. 1989; 97(3): 326-330.
Walker, Q.O and Mailman,R.B. Triadimefon and Triadimenol: effects on monoamine uptake and release. Toxil.Appl.Pharmacol. 1996; 139 (2): 227-235.
Moser, V.C; Cheek, B.M.and MacPhail,R.C. Multidisciplinary approach to toxicological screening. III Neurobehavioral toxicity:. Toxil. Enoviron. Heal. 1995;45:2,173-210.
Allen, A.R and MaCphail, R. C. Ritertanol, triazole fungicide, increase operant responding but not mortality: Neurotoxicol. teratol. 1993;15 (4):237-242.
Sahar, M.A.N; Siddig, K.E; Barri, M.E; Abdel Salam, E.B; and Mohamed, O, S.A. Toxicity of Diazinon in Nubian goats. Sud.J.Vet.Sc.Anim.Husb. 1998; 37(1,2), 76-83.
Silva-M.do,R.R ;Paiva-LF-de; Jesino-R-SA. and De-PaviaL.F.C. (1994). Evaluation of Ketoconazole in mice inoculated with Paracooidiodes brasiliensis thruogh liver and spleen histopathology and by Paracooidiodin rdemal reaction : Revistad a- Sociedadei-de-medicina-Tropical 27 (1 ) , 11-14.
Doeglas, H.M.B. Antifungal drugs in Side effects of drugs Annual 3-(1979) .Excerpt MEDICA 1979; vol.(2):pp.236-238.
FAO/WHO. (1986). Triadimefon, FAO plant production and protection paper; 72/2:199-202.
Ammerman, C.B; Chicco, C.F.; Moore, J.E.; Van Walleghem, P.A. and Arrington, L.R. (1971). Effect of dietary magnesium on voluntary food intake and rumen fermentations. Journal of Dairy Science (45): 1288-1293.
Bell, F.R. (1995). Perception of sodium and sodium appetite in farm animals. In:Philteps, C.J.C. Agriculture. Chalcembe Publications, Canterbury UK, pp82-90.
Rays, H.P and Dale, M.M.(1991).Pharmacology. Living stone (pupl.). pp 274.
