Methylenetetrahydrofolate Reductase (MTHFR C677T) Gene Polymorphism in Sudanese Patients with Acute Lymphocytic Leukemia

د.عبد العظيم; Mahdi H.A.  Abdalla

doi:10.52981/smlj.v12i1.3127

د.عبد العظيم Department of Hematology, Faculty of Medical Laboratory Sciences, Alneelain University, Sudan
Mahdi H.A. Abdalla Department of Hematology, Faculty of Medical Laboratory Sciences, Omdurman Ahlia University, Sudan

DOI: https://doi.org/10.52981/smlj.v12i1.3127

الملخص

Background: Acute lymphocytic leukemia is one of the major types of leukemia that are found in Sudan. This study aimed to examine the associations of MTHFR c677T polymorphism with the risk of acute lymphocytic leukemia among patients in Sudan.

Methods: A total of 50 patients with myocardial infarction and 50 healthy controls who were matched

by age and gender were included in the study. MTHFR C677T polymorphism was studied in both cases and healthy controls, 2 ml of blood samples were collected from both patients and controls into EDTA anticoagulated tubes, the extraction of DNA was done using the salting out approach, The primers, were used to amplify the MTHFR C677T fragment, the amplified fragment was detected on an agarose gel electrophoresis. And count the blood cells.

Results: low frequency of the mutants MTHFR C677T genotype with CT genotype being present in

6.7% of the patients and CC genotype representing 93.3% of the population. Among healthy controls,

the frequency of CT genotype was 8% while that of CC genotype was 90%. There was no significant

association between MTHFR gene polymorphism and the risk of ALL.

Conclusion: Our data suggest a low impact of MTHFRC677T gene polymorphism on the of developing ALL.

المراجع

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