Serological and molecular diagnosis of toxoplasmosis in pregnant women in
Keywords:
molecular diagnosis, Serological diagnosis, toxoplasmosis, pregnant women
Abstract
This study was conducted to determine the sero-diagnosis and risk factors of toxoplasmaosis among pregnant women in Omdurman, Khartoume state, during the period of June 2006 to July 2008. The study included 455 pregnant women between 25 – 35 years of ages.
The blood samples were collected and examined by direct latex agglutination, ELISA IgG and IgM and ten by PCR test. All serological investigations were carried out in the center for research and laboratory sciences, Faculty of Medicine, Ain Shams University, Egypt. Data were collected from the pregnant women by interview using modified questionnaire.
The results showed that the prevalence of Toxoplasma gondii was 17.8% by direct latex agglutination, 38.9% by ELISA IgG (indicating chronic infections) and 12.9% by IgMindicating acute infections. The results of PCR test showed 16.7%. Although of questionable accuracy, the results of the present study revealed a relatively high sero-prevalence of acute infection in the pregnant women, with Toxoplasma
References
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14. Adnan, El Hindy. (1994). Sero-epidemiology of human of human toxoplasmosis and internal Parasites of cats in Khartoum. A thesis submitted for the degree of Master of Science in zoohogy. Faculty of sciences. University of Khartoum.
15. Kimball AC, Kean BH, Fuchs F. (1971). Congenital toxoplasmosis: a prospective study of 4,048 obstetric patients. Am J Obstet Gynecol; 111:211-8.
16. Maha H. Elshaikh (2006) Seroprevalence by IgG and IgM and Molecular charactriziation of Toxoplasmosis during pregnancy in Central Sudan.Department of Pathology .Universty of Gazira.
17. Elfakahani, A.F., Abdel-Mabound, AL., Elgarhy, M.F. and Eraky, M.A. (2002). Comparitive study between ELISA IgG, IgM, and PCR in diagnosing and studying toxoplasmosis in Qualyobia Governorate, Egypt. Egypt Soc Parasitol J.32(2):475-486.
18. Bornandm J.E; and Gottrau, P. (1997) Uveitis: Is ocular toxoplasmosis only a clinical diagnosis?. Ophthamologica. 11:87-89.
19. Burnett, A. J; Shortt, S.G; Isaac-Renton, J; King, A; Werker, D. and Bowie, W.R., (1998). Multiple cases of acquired toxoplasmosis retinitis presenting in an outbreak. Ophthalmology. 105(6):1032-1037.
20. Cao.Y,Qiu L,Zhang Q,(1999). Study on the relationship between the history of abnormal pregnancy ant TORCH infection in pregnant woman.Zhonghua Fu ChanKeZa Zhi:34:514-520.
21. Elawady, M. K., Elhosseiny. L.A., Ismail. S.M., Abdulaziz. M.T. and Eldemellawy, M.A. (2000). Comparison between toxoplasma gondii DNA and specific immunoglobulins during pregnancy. Eastern Mediterranean Health J. 6 (5).
22. Mei-Huilin, L., Tse-Ching, C., Tseng-Tong, K., Ching-chung, T. and Ching-ping, T. (2000). Real time PCR for quantitative detection of Toxoplasma gondii. Jornal of Clin. Microbiol. 38(11):4121-4125.
23. Pinon, J.M., dumon, H. and Chemla, C. (2001). Strategy for diagnosis of congenital texoplasmosis: evaluations of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G, M, and antibodies. J Clin Micobiol. 39:2267-2271.
24. Jones, J.L. Moran, K.D. and Wilson, M (2003). Toxoplasma gondii infection in the United States, 1999-2000 Emerg Infect Dis 9(11): 1371-1374.
25. Liesenfeld, O., Montoya, J.G. and Tathyinemi, N. J. (1997). Confirmatory serological testing results in remarkable decrease in unnecessary women of history with miscarrage among pregnant women in the United States with positive toxoplasma serology. (Abstract). 35th Annual Meeting of the Infectious diseases Society of America. San Franciso, Calif: 76.
26. Aslanzadeh, J. (2004). Preventing PCR amplification carryover contamination in a clinical laboratory. Ann.Clin.Lab.Sci. 34(4):389-396.
27. Romand, S., Wallon, M., Franck, J., Thulliez, P., peyron, F. and Dumon, H. (2001). Prenatal diagnosis using polymerase chain reaction on amniotic fluid for congenital toxoplasmosis. Obstet Gynecol 97(2):296-300.
28. Neto, E.C., Rubin, R., Schulte, J. and Giugliani, R. (2004). Newborn screening for congenital infectious diseases. Emerg Infect Dis. 10(6).
2. Carter, F.S; and Fleck, D.G. (1966). The incidence of toxoplasma antibodies in Sudanese. Transaction of the Royal Society of Tropical Medicine and Hygiene. 60: 539-543.
3. Abd Elhameed, A.A. (1991). Sero-epidemiology of toxoplasmosis in Gezira- Sudan. J.Trop. Med.Hyg. 94:329-332.
4. Elnahas, A.H. (1997). Sero- epidemiology of toxoplasmosis in Sudanese pregnant women in Khartoum State.. A thesis submitted for the degree of clinical M.D. In: Obstetrics and Gynecology. Faculty of Medicine. University of Khartoum.
5. Remington, J.S; Meleod, R; and Demonts, G. (1995). Toxoplasmosis In: Remington, J.S. and Klein, J.O. Infectious diseases of the fetus and newborn 4th ed. Pennsylvania:WB Saunders. 140-267.
6. Jenum, P.A; Stray-Petersen, B; and Guandersen, A.G. (1997). Improved diagnosis of primary toxoplasma infection in early pregnancy by determination of anti-toxoplasma immunoglobulin G. avidity. J clin Microbiol. 35:1972.
7. El Hady HM. (1991). Toxoplasmosis among pregnant women in Abha, Saudi Arabia. J Egypt Soc Parasitol. 21:811–815.
8. El Ridi, A.M; Nada, S.M; Aly, A.S; Habeeb, S.M; and Abdul-Fattah, M.M. (1990). Serological studies on toxoplasmosis in Zagazig slaughterhouse. J. Egy. Soc. Parasitol. 20, 677–681.
9. Abdelhafez, S.K; Shbeeb, I.I; and Abdelrhmman, F. (1986). Serodiagnosis of T.gondii in Habital aborting women and other adults from North Jordn. Folia.Parasitol. 33:7-13.
10. Qublan, H.S., Junian, N., Abu-Salem A., Hamadelil, F.Y., Mashagbeh, M. and Abd Elghani, F. (2002). Toxoplasmosis and habitual women of history with miscarrage. J.Obstet Gynecol. 22(3):296-298.
11. Ashrafunnessa, S; Khatun, M.N; and Islam, T Huq. Seroprevalence of toxoplasma antibodies among the antenatal population in Bangladesh. J Obstet Gynaecol Res. 1998 Apr; 24 (2):115-9 9631599
12. Sahwi, S.Y., Zaki, M.S., Hayba, N.Y., Elsaid, O.K., Anwar, M.Y., and Abd-Rabbo, S.A. (1995). Toxoplasmosis as a cause of repeated women of history with miscarrage. J Obstet Gynecol. 21(2):145-148.
13. Leo, P. R., Meirelles, F.J., and Medeiros, S.F. (2004). Toxoplasmosis Seroprevalence in postpartum women. (Brazilian Public Health System). Rev Bras Gynecol Obstet. 26 (8): 623-627.
14. Adnan, El Hindy. (1994). Sero-epidemiology of human of human toxoplasmosis and internal Parasites of cats in Khartoum. A thesis submitted for the degree of Master of Science in zoohogy. Faculty of sciences. University of Khartoum.
15. Kimball AC, Kean BH, Fuchs F. (1971). Congenital toxoplasmosis: a prospective study of 4,048 obstetric patients. Am J Obstet Gynecol; 111:211-8.
16. Maha H. Elshaikh (2006) Seroprevalence by IgG and IgM and Molecular charactriziation of Toxoplasmosis during pregnancy in Central Sudan.Department of Pathology .Universty of Gazira.
17. Elfakahani, A.F., Abdel-Mabound, AL., Elgarhy, M.F. and Eraky, M.A. (2002). Comparitive study between ELISA IgG, IgM, and PCR in diagnosing and studying toxoplasmosis in Qualyobia Governorate, Egypt. Egypt Soc Parasitol J.32(2):475-486.
18. Bornandm J.E; and Gottrau, P. (1997) Uveitis: Is ocular toxoplasmosis only a clinical diagnosis?. Ophthamologica. 11:87-89.
19. Burnett, A. J; Shortt, S.G; Isaac-Renton, J; King, A; Werker, D. and Bowie, W.R., (1998). Multiple cases of acquired toxoplasmosis retinitis presenting in an outbreak. Ophthalmology. 105(6):1032-1037.
20. Cao.Y,Qiu L,Zhang Q,(1999). Study on the relationship between the history of abnormal pregnancy ant TORCH infection in pregnant woman.Zhonghua Fu ChanKeZa Zhi:34:514-520.
21. Elawady, M. K., Elhosseiny. L.A., Ismail. S.M., Abdulaziz. M.T. and Eldemellawy, M.A. (2000). Comparison between toxoplasma gondii DNA and specific immunoglobulins during pregnancy. Eastern Mediterranean Health J. 6 (5).
22. Mei-Huilin, L., Tse-Ching, C., Tseng-Tong, K., Ching-chung, T. and Ching-ping, T. (2000). Real time PCR for quantitative detection of Toxoplasma gondii. Jornal of Clin. Microbiol. 38(11):4121-4125.
23. Pinon, J.M., dumon, H. and Chemla, C. (2001). Strategy for diagnosis of congenital texoplasmosis: evaluations of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G, M, and antibodies. J Clin Micobiol. 39:2267-2271.
24. Jones, J.L. Moran, K.D. and Wilson, M (2003). Toxoplasma gondii infection in the United States, 1999-2000 Emerg Infect Dis 9(11): 1371-1374.
25. Liesenfeld, O., Montoya, J.G. and Tathyinemi, N. J. (1997). Confirmatory serological testing results in remarkable decrease in unnecessary women of history with miscarrage among pregnant women in the United States with positive toxoplasma serology. (Abstract). 35th Annual Meeting of the Infectious diseases Society of America. San Franciso, Calif: 76.
26. Aslanzadeh, J. (2004). Preventing PCR amplification carryover contamination in a clinical laboratory. Ann.Clin.Lab.Sci. 34(4):389-396.
27. Romand, S., Wallon, M., Franck, J., Thulliez, P., peyron, F. and Dumon, H. (2001). Prenatal diagnosis using polymerase chain reaction on amniotic fluid for congenital toxoplasmosis. Obstet Gynecol 97(2):296-300.
28. Neto, E.C., Rubin, R., Schulte, J. and Giugliani, R. (2004). Newborn screening for congenital infectious diseases. Emerg Infect Dis. 10(6).
Published
2022-10-15
Section
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