Association between Helicobacter pylori infection and upper gastrointestinal symptoms in Sudanese patients, Khartoum state- Sudan

AbdulAzeem AbdulSalam Ibrahim  Alkhidir; Arwa  Algaili; Asma  Ahmed; Baraa  Yousif; Eslam  Ahmed; Fatima Alzahra  Nsraldeen; Alzahra  Abdalkhalige

doi:10.52981/smlj.v12i1.3124

AbdulAzeem AbdulSalam Ibrahim Alkhidir 1Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan. - Department of Microbiology, Faculty of Medicine, Omdurman Ahlia University, Sudan.
Arwa Algaili Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan.
Asma Ahmed Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan.
Baraa Yousif Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan.
Eslam Ahmed Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan.
Fatima Alzahra Nsraldeen Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan.
Alzahra Abdalkhalige Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan.

DOI: https://doi.org/10.52981/smlj.v12i1.3124

Keywords: H.pylori, Stomach biopsy, Endoscopy

Abstract

Introduction: Helicobacter pylori is small, spiral, Gram-negative bacilli that plays a role in the pathogenesis of a number of diseases, is strongly associated with gastric cancer and peptic ulceration. The bacterium highly links to duodenal ulcer, which was classified as a group I carcinogen in 1994 by the WHO.

Objectives-This study aimed to isolate Helicobacter Pylori from stomach biopsy, and to assess the correlation between H.pylori infection and upper gastrointestinal symptoms (Epigastric pain, and hunger) and Duodenitis.

Material and Methods: This is a descriptive cross-sectional study.Out of 40 participants were included in this study. The antral mucosal biopsy specimens were obtained. According to standard microbiology procedure the specimens were cultured on Modified Brain heart Hemoglobin Urea Agar which is selective and differential media for H. pylori, the urease activity of H.pylori observed within 24 hours in this media .while, the growth were observed after 3 days following the incubation of cultured plates under microaerophilic condition provided by candle jar.

Result: H. Pylori was detected in 2(5%) of 40 stomach biopsy specimens. And this study revealed that there is no association between H.pylori infection and Epigastric pain, hunger pain and Duodenitis.

Conclusion-This study concludes that there is no association between H.pylori infection and upper gastrointestinal symptoms (epigastric pain and hunger) and duodenitis.

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